Sustainable cycle-tourism for society: Integrating multi-criteria decision-making and land use approaches for route selection

Cycle tourism is a sustainable active vacation, which is quickly growing in recent years. Although it has several benefits for society and users (e.g., social connections, amusement, and physical and mental health), cycle tourism requires an adequate route network to enjoy destinations with historical and landscape peculiarities. Past literature mainly investigated motivations and preferences for cycle tourists and proposed optimisation methods in planning routes. However, applying assessment methods for prioritising cycle-tourist routes is a seldom-explored topic. This study aims to address this gap by applying an integrated method for evaluating and prioritising cycle routes, searching for a compromise between route characteristics, service provided to users, and natural and building contexts crossed. It jointly includes Multi-Criteria Decision Methods (MCDMs) and a land use approach: AHP determines the weights of criteria and parameters describing cycle routes; GIS elaborates spatial analysis of parameters; ELECTRE I and VIKOR help find a compromise solution amongst different cycle routes. The integrated method involved a panel of experts to collect data, and it is applied to the wide-study area of Franciacorta (Italy). Some comparisons with other MCDMs are made to justify the results. The findings could support multi-institutions prioritising cycle route alternatives in deciding their building

Review of combustion indexes remote sensing applied to different combustion types

This paper summarizes the main studies carried out by the authors for the development of indexes for remote combustion sensing applicable to different combustion types, i.e. conventional gasoline and diesel combustions, diesel PCCI and dual fuel gasoline-diesel RCCI. It is well-known that the continuous development of modern Internal Combustion Engine (ICE) management systems is mainly aimed at complying with upcoming increasingly stringent regulations throughout the world, both for pollutants and CO2 emissions. Performing an efficient combustion control is crucial for efficiency increase and pollutant emissions reduction. Over the past years, the authors of this paper have developed several techniques to estimate the most important combustion indexes for combustion control, without using additional cylinder pressure sensors but only using the engine speed sensor (always available on board) and accelerometers (usually available on-board for gasoline engines). In addition, a low-cost sensor based on acoustic sensing can be integrated to support combustion indexes evaluation and other engine relevant information. The real-time calculation of combustion indexes is even more crucial for innovative Low Temperature Combustions (such as diesel PCCI or dual fuel gasoline-diesel RCCI), mainly due to the high instability and the high sensitivity to slight variations of the injection parameters that characterize this kind of combustions. Therefore, the authors of this paper have applied the developed techniques not only to conventional engines (gasoline and diesel combustion), but also to engines modified for Low Temperature Combustions, with promising results in terms of validation and applicability for real-time combustion control. The developed methodologies have been tested and validated through a large amount of experimental tests. To run the estimation algorithms in real-time, they have been all implemented in a specifically designed rapid control prototyping system, the goal being to quantify the accuracy of the estimations and optimize the strategy implementations for the extensive use (in the near future) in modern Engine Control Modules (ECM)

A comparative cycling path selection for sustainable tourism in Franciacorta. An integrated AHP-ELECTRE method

Cycle tourism is a form of sustainable itinerant tourism expanding in Italy and the rest of the world, with prospects for growth in coming years. Europe and North America have already developed a wide range of cycling infrastructures tied to tourism experiences. Benefits induced are generally recognised: first, it is a sustainable solution that increases local economics while conserving the environment; second, it guarantees advantages on social connections, amusement, and physical and mental health. However, it requires an adequate network to enjoy destinations as historical and landscape peculiarities. Currently, literature provides some methods for planning itineraries dedicated to cycle tourism. Despite that, there is less attention on how evaluating existing or already planned tourist itineraries. This study covers this gap, by applying an integrated method to assess bicycle connections for tourism experiences within municipalities. Since this evaluation may contain many conflicting criteria (e.g., preferences of public administrator, technical and economic viability) and possible alternatives, this study frames the method as a multi-criteria decision-making problem (MCDM). Specifically, at first, the Analytical Hierarchy Process (AHP) is adopted to calculate weights for each criterium; next, the ELimination Et Choix Traduisant la REalitè (ELECTRE) method is applied to provide a (possible) priority ranking of cycling tourist paths among alternatives, by computing indices of discordance and concordance between pairs of alternatives. The framework is applied to the Franciacorta area (North-East Italy), a national and international tourist relevance territory encompassing 22 municipalities. This study may be useful for public administrators to rationalise and prioritise cycling routes

Shared detection of Porphyromonas gingivalis in cohabiting family members: a systematic review and meta-analysis

Introduction: Periodontitis is an inflammatory dysbiotic disease. Among putative dysbiosis causes, transmission of Porphyromonas gingivalis between individuals of the same family remains unclear. The aim of this systematic review and meta-analysis is to assess the likelihood of shared detection of Porphyromonas gingivalis among cohabiting family members. Methods: A literature search?was conducted on different databases up to September 2018. Articles assessing the presence of P.gingivalis between members of the same family were screened. Only English literature was retrieved, whereas no limits were applied for bacterial sampling and detection methods. Results: Overall, 26 articles published between 1993 and 2017 met the inclusion criteria. Of these, 18 articles were used for meta-analyses. Based on bacterial culture, the likelihood of an intra-familial transmission of P.gingivalis once a member of the family harbors the bacterium is estimated at 63.5% (n = 132 pairs of family members); this drops to 45% when pooling together culture and Polymerase-Chain-Reaction (n = 481 pairs), whereas it is estimated at 35.7% when genotyping is applied (n = 137 pairs). Conclusion: Pooled results suggest that the likelihood of detecting P.gingivalis within within family members is moderately frequent. Personalized periodontal screening and prevention may consider intra-familial co-occurrence of P.gingivalis as feasible

Influence of Culture Conditions on In Vitro Asymbiotic Germination of Anacamptis longicornu and Ophrys panormitana (Orchidaceae)

This study is the first approach to in vitro asymbiotic germination of two species of Sicilian threatened terrestrial orchids, Anacamptis longicornu and Ophrys panormitana. Seeds were collected in the wild and cultured in two different media—Orchimax medium (OM) and Murashige and Skoog (MS)—and exposed to different photoperiods and temperatures to evaluate the best conditions for the specific stages of development. The germination of A. longicornu was very high on OM (95.5%) and lower on MS medium (21.4%), whereas O. panormitana germinated only on OM medium, with significantly lower percentages (12.0%), compared with A. longicornu. This difference is caused by variation in quality and quantity of nutrients used, primarily by nitrogen source. The results show that temperature and photoperiod widely affect seed germination and development. Although further investigations on asymbiotic and symbiotic germination are needed for the improvement of conservation of Mediterranean terrestrial orchids, our results contribute to the conservation of this group of plants

Defective ribosomal products challenge nuclear function by impairing nuclear condensate dynamics and immobilizing ubiquitin

Nuclear protein aggregation has been linked to genome instability and disease. The main source of aggregation-prone proteins in cells is defective ribosomal products (DRiPs), which are generated by translating ribosomes in the cytoplasm. Here, we report that DRiPs rapidly diffuse into the nucleus and accumulate in nucleoli and PML bodies, two membraneless organelles formed by liquid\u2013liquid phase separation. We show that nucleoli and PML bodies act as dynamic overflow compartments that recruit protein quality control factors and store DRiPs for later clearance. Whereas nucleoli serve as constitutive overflow compartments, PML bodies are stress-inducible overflow compartments for DRiPs. If DRiPs are not properly cleared by chaperones and proteasomes due to proteostasis impairment, nucleoli undergo amyloidogenesis and PML bodies solidify. Solid PML bodies immobilize 20S proteasomes and limit the recycling of free ubiquitin. Ubiquitin depletion, in turn, compromises the formation of DNA repair compartments at fragile chromosomal sites, ultimately threatening cell survival

Ph-positive CML in blastic phase with monosomy 7 in a Down syndrome patient. Monitoring by interphase cytogenetics and demonstration of maternal allelic loss

We report a case of Ph-positive chronic myelocytic leukemia in blastic phase in an 11-year-old boy with Down syndrome. Monosomy 7 was the only additional chromosomal anomaly in the blastic clone. Fluorescence in situ hybridization analysis on interphase nuclei with a centromeric probe specific to chromosome 7 proved to be efficient in disease monitoring; and showed, together with the results of chromosome analysis on metaphases, that B- lymphocytes at the origin of an EBV-established line were not part of the leukemic clone. The study of DNA polymorphisms showed that the origin of the constitutional trisomy 21 was a maternal anaphase I nondisjunction, that the chromosome 7 lost in the blastic marrow clone was the maternal one, and led us to postulate that the mother's chromosomes are prone to impairment of normal disjunction. The study of allelic losses of chromosome 7 loci proved to be a further possibility for disease monitoring

Effect of atmospheric nitric oxide (NO) on measurements of exhaled NO in asthmatic children

The measurement of exhaled nitric oxide concentrations [NO] may provide a simple, noninvasive means for measuring airway inflammation. However, several measurement conditions may influence exhaled NO levels, and ambient NO may be one of these. We measured exhaled NO levels in 47 stable asthmatic children age 5 to 17 years and in 47 healthy children, gender and age matched. Exhaled [NO] in expired air was measured by a tidal breathing method with a chemiluminescence analyzer, sampling at the expiratory side of the mouthpiece. NO steady\u2010state levels were recorded. In order to keep the soft palate closed and avoid nasal contamination, the breathing circuit had a restrictor providing an expiratory pressure of 3\u20134 cm H2O at the mouthpiece. To evaluate the effect of [NO] in ambient air, measurements were randomly performed by breathing ambient air or NO\u2010free air from a closed circuit. Breathing NO\u2010free air, exhaled [NO] in asthmatics (mean \ub1 SEM) was 23.7 \ub1 1.4 ppb, significantly higher (P < 0.001) than in healthy controls (8.7 \ub1 0.4 ppb). Exhaled NO concentrations measured during ambient air breathing were higher (49 \ub1 4.6 ppb, P < 0.001) than when breathing NO\u2010free air (23.7 \ub1 1.4 ppb) and were significantly correlated (r = 0.89, P < 0.001) with atmospheric concentrations of NO (range 3\u2013430 ppb). These findings show that (1) exhaled [NO] values of asthmatic children are significantly higher than in healthy controls, and (2) atmospheric NO levels critically influence the measurement of exhaled [NO]. Therefore, using a tidal breathing method the inhalation of NO\u2010free air during the test is recommended. Pediatr Pulmonol. 1998; 26:30\u201334. \ua9 1998 Wiley\u2010Liss, Inc